Clinical Specimen Submission Guidance

Submit all specimens to 50 Orms St, Providence RI 02915. Open Monday to Friday 8:30 AM to 4:30 PM

Influenza Virus

Lab Molecular Diagnostics Laboratory 401-222-5538
Organism Influenza Virus
Disease Influenza
Test Description

Real-Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR) for the detection of Influenza RNA

Special Instructions

Specimens are only accepted from designated RI Sentinal Sites that are approved by the RIDOH Center for Acute Infectious Disease Epidemiology (401) 222-2577.

All other laboratories must obtain PREAUTHORIZATION by the RIDOH Center for Acute Infectious Disease Epidemiology (401) 222-2577 prior to transporting specimens.

Specimen

Refer to the Influenza Specimen Collection Kit Instructions for specimen collection.

Specimen Type:

Collect nasopharyngeal specimens with a flexible wire shaft nasopharyngeal (NP) swab.

Collect throat specimens with a plastic shaft swab with a polyester, Dacron or rayon tip

The NP swab and the throat swab can be placed in the same tube of VTM

After specimen is obtained, inoculate the tube of Viral Transport Media (VTM) by inserting the swab into the tube making certain that the liquid in the tube covers the swab tip. The shaft should be clipped equal with the top of the tube.

Store refrigerate at 2-8C for up to 72 hours. If unable to transport within 72 hours, the specimen must be frozen at -70C.

CLIA regulations require two patient identifiers on the specimen container and the test requisition

Form Required

Rhode Island State Health Laboratories (RISHL) test requisition form.

Test Request

Write "FLU" in comment field on requisition form.

Transport

Specimens must be transported on wet ice or equivalent cooling material and packaged in accordance with current federal shipping regulations.

If specimen previously frozen it must be transported on dry ice.

Containers specifically labeled for transport of specimens to the RISHL Molecular Diagnostics Laboratory are available in all microbiology labs and send out departments.

Normal Value PCR Negative for Influenza A and B
Turnaround Time PCR result within 48 to 96 hours